For optimal growth, Ajuga ophrydis must be planted in a sunny position, in a well-drained and composted loam soil. It should receive summer rainfall, with moderate water during the spring and summer growing season, and kept dry during winter. It is an ideal addition to a grassland garden and should be inter-planted with grass species, and can also be used as a groundcover and in herbaceous mixed borders. It should not be disturbed, once planted.

Propagation can be done by division of the plants, by separating the underground rhizomes, as well as by soft, tip cuttings taken in spring and summer. They can also be grown from seed, although seed is difficult to obtain, and consequently most plants are propagated by cuttings. Cuttings should be made in summer from November to February; each cutting should comprise several nodes and the leaves removed. They should be planted a ⅓ deep in well drained, clean sand and kept in a well-lit position, but not direct in sunlight. Cuttings should be watered daily until rooted. The use of rooting hormone is recommended. Cuttings can remain green throughout the winter months, by keeping them in a warm greenhouse and continuing with watering. Rooted cuttings can be planted into individual containers in their second season of growth. Feed with an organic fertilizer.

No significant insects or disease problems have been noted for this species in cultivation, but crown rot can be a problem, usually in humid conditions and waterlogged soil.