Position Aloe mudenensis in an area that gets full sun for at least half the day. The area must also have good drainage, such as a slope, a north-facing slope being the best.

Despite Aloe mudenesis being a hardy plant, it still requires nutrients to thrive. The best way to ensure healthy plants is to prepare the soil correctly with organic compost before planting and then feed every few years. If Aloe mudenensis becomes too dry, the leaves will wrinkle up and the colour will fade. Regular irrigation, especially in dry weather, will keep them lush, however, if they are exposed to too much water, the leaves will drop or the whole plant will rot.

They can also be propagated from seeds which germinate easily, if sown in well-drained soil and lightly covered with fine sand.