Barleria heterotricha subsp. heterotricha can be propagated by seed or cuttings.

To prevent seed being lost it can be collected as the capsules turn brown. The seeds should be planted in a mixture of two parts good soil and one part clean river sand. Place them in a shady spot in your garden and water once a week. Germination of the seed takes place after a week. Transplanting the seedlings into bags should be done when the seedlings are more or less 100 mm high.

Cuttings should be done in summer. Make use of plant material of the previous year's growth. Take cuttings of 120 mm. Treat them with a hormone powder and plant them in a mixture of even parts good soil and river sand. Put the cuttings in a shady place and water twice a week. Transplant the rooted cuttings after about three months into a good soil mixture.