Cotyledon gloeophylla is an attractive plant with horticultural appeal. It is characterized by its decorative, sticky, flat, green leaves and pendent tubular flowers. It grows well in cultivation and does best as a containerized plant and is suitable for window sills, or it can be grown in rockeries. In cold climates where frost occurs it should rather be grown in a greenhouse under controlled conditions. It is a slow grower, but eventually forms a small branched shrublet. Cotyledon gloeophylla is best cultivated in Thicket and Succulent Karoo Gardens (Van Jaarsveld 2010), or similarly dry Mediterranean-type gardens in other parts of the world, where frost is not too severe. Plants prefer full sun to partial shade. If grown in shady conditions, the leaves tend to be larger and greener. Flowering size can be reached in about 3 to 4 years.

Cotyledon gloeophylla is easily propagated from stem cuttings or seed. Cuttings are best taken in spring or summer and rooted in a small container in a well-drained medium such as sand, but can take several weeks to root. Sow seed preferably during spring or summer in a shady position in a sandy, slightly acidic, soil. Cover with a thin layer of sand and keep moist. Best to feed plants with an organic fertilizer, compost or any other liquid fertilizer. Cotyledon gloeophylla can be watered at any time of the year, its main growing season is summer and watering should be reduced in winter.