All gasterias thrive in cultivation and are suited to both indoor and outdoor cultivation. Gasteria bicolor var. liliputana can be used as a pot plant on window sills,verandas, in a miniature succulent garden where they are happy to share their habitat with other smaller succulent plants, or in outdoor rockeries. They are tolerant of a wide range of soils and habitats, but care must be taken in areas where rainfall is high or frost is frequent. They prefer partial or dappled shade and outdoors are best planted on the shady side of a rock, or in the shade of other plants.

This plant is drought tolerant and will grow well in the gardens of the winter rainfall Western Cape, provided it is planted in well-drained soil and is occasionally moistened during the dry summer months. As with many other succulent plants, leaves may turn red if the plant is stressed. It is not shy to flower and will flourish if fed with liquid organic fertiliser.

Gasteria bicolor var. liliputana is easily propagated from division, leaf cuttings or seed. To get seed from gasterias in cultivation, they must be cross pollinated. To pollinate, mimic the action of a sunbird, i.e. find a small stick, like a matchstick, gently insert it into a flower on one plant, then transfer the pollen that rubbed off onto the stick, onto the stigma of a flower on another plant. Capsules abort very soon if the flowers are not pollinated. Seed should be sown during spring or summer, in a sandy, well-drained potting soil, covered with a thin layer of sand (1-2 mm), placed in a warm shady position and kept moist. Germination occurs within 3 weeks and the seedlings are slow growing. The seedlings can be planted out in individual bags as soon as they are large enough to handle. First flowering can be within 3-4 years.

To propagate by leaf cuttings, remove a leaf and let it lie for about one month, giving the wound time to heal. Then lay the leaf on its side with the basal part buried in the soil. This leaf should root within a month or two, and small plants will form at the leaf base. These can be removed and potted up as soon as they are large and firm enough to handle. A clump of Gasteria bicolor var. liliputana can be divided at any time of the year.