Gladiolus longicollis is seldom cultivated. It is not a showy species, but is an interesting addition to a grassland garden or sunny rockery, and is suitable for containers.

It can be cultivated from corms as well as seeds. It should preferably be planted in light, well-drained, sandy loam soil in full sun. As this is a summer-growing species, plant corms in spring (around September) and give plenty of water all through the growing season. Corms are best planted in open ground, rather than pots, roughly 10 cm deep and 15 cm apart. Avoid the use of inorganic fertilizers, especially those with high nitrogen content. Rather use organic compost or bone meal. The corms can be left in the soil during the winter dormancy period, or lifted and stored in a cool airy place to prevent decay.

When growing from seed, sow in September. Use a well-drained, sterile sowing medium, sow the seeds thinly and cover lightly. The seeds generally germinate well and seedlings appear after 2 to 4 weeks. Water thoroughly all through the growing season. However, flowers will only be produced from the second season onwards.