Haworthiopsis limifolia grows best in a semi-shaded position, under big succulent plants, like Aloe or Euphorbia, or planted in a rock garden in between rocks, where they will form a clumps over time. Direct sun causes sunburn and may result in leaves turning white or yellow.

Haworthiopsis limifoila is a drought-tolerant plant, it will grow in frost-free, arid areas and is sensitive to overwatering. Damping off can occur if plants are overwatered or the soil medium is waterlogged. Soil must be well-drained, sandy, with a lot of organic matter. Geoff Nichols recommends using 1:1 mixture of river sand and compost. In the beginning of spring, use a sprinkling of hoof and horn fertiliser on the soil surface. In the summer months, from the beginning of September to the end of March, feed the plants weekly with a liquid fertiliser. As a pot plant indoor, place the plant in a position that allows it to get lots of light throughout the day, without constant direct sunlight.

Haworthiopsis limifolia can be propagated using three methods: seeds, division and leaf cuttings.  Seed are sown in spring in well-drained soil and covered lightly. For quick germination, place the seeds on a mist bed with bottom heat at 25ºC. Seeds will germinate after about 5 to 10 days and initially grow quite slowly. After about a month, the leaves are produced. According to Geoff Nichols H. limifoila has recalcitrant seeds and should be sown as soon as possible, after 3 weeks the viability of the seed will drop considerably. Seeds should be planted as soon as the capsule turns a straw colour and begins to split. One can speed up the process by manually splitting the capsules to release the seeds.

Small offsets appear naturally around the base of the mother plant, these offsets can be removed when they are large enough to handle, in spring or summer. Allow these offsets to dry for 1 or 2 days, before replanting in individual pots, into a well-drained mixture of river sand and bark.

To propagate from leaf cuttings, remove leaves from the main plant by gently twisting the leaf from the stem. Allow the leaf to dry for several days to form a callus over the wound, and plant them in moist sand.

There are no major pests of diseases recorded for this species, however if plants are potted into too large a container, the soil will become sour and, owing to too much moisture being retained in the soil, the roots will rot off. Species in this genus like to dry out between watering. If the plants lose vigour, mealy bug will attack the young roots and soft tissue at the base of each leaf. Soaking the plant in a Malathion bath is the best treatment (Nichols 2005).