Barleria matopensis can be propagated either by seed or cuttings.

Collect the seed as the capsules turn brown. Open the capsules and harvest the seed. Fertile seeds are well developed. Sow the seeds in a mixture of equal parts of coarse river sand, soil and compost in late spring or in summer (October–March). Place the container with seed in a shady spot and water once a week. Germination takes place after 6–10 days. Transplant the seedlings when they are 100 mm tall.

Take semi-hardwood cuttings, 120 mm long, treat the cuttings with a hormone powder to stimulate root growth. Use a mixture of even parts washed river sand and compost as a growing medium. Put the container in a cool, shady place and water twice a week. Transplant the rooted cuttings after 4 months. Expect a success rate of 60%.

Barleria matopensis is a water-wise plant, growing well in sandy and loam soils. Use it in combination with plants like Aloe marlothii (mountain aloe) and A. cryptopoda (yellow aloe) and Barleria rotundifolia (spiny yellow barleria).

Acknowledgements: the author thanks Dr Hugh Glen, who translated the original description of Barleria matopensis from Latin to English for the author.