The plants in this genus are relatively easy to cultivate. In their natural habitat, they experience their main growth period in late winter and enter a period of dormancy in spring and summer. However, in cultivation it is recommended to provide them with some water throughout the year to prevent their epidermis from breaking and causing unsightly scars. During the middle of summer to the end of winter, it is advisable to water them moderately, while keeping the soil almost dry when the plants are dormant. In spring and summer, water should be given minimally, only when the plant starts to shrivel. Although they can still grow in summer if given water, it is generally best to water them sparingly during this time. If you live in an area prone to frost, it is recommended to grow them in a well-ventilated greenhouse or conservatory, using well-drained sandy loam soil suitable for succulent plants. During summer, keep the plants cool and shaded, but provide maximum light for the rest of the year. It is important to note that these plants thrive in sunny locations, as insufficient sunlight may affect their ability to flower properly.

There are two approaches to growing glottiphyllums. The first involves allowing nature to take its course by using large pots and providing ample water, resulting in abundant green leaves. The second approach involves simulating their natural habitat by intentionally subjecting them to hot summer droughts, using small pots and firm loam to achieve a more slender appearance. Both methods can yield attractive results. Regardless of the approach chosen, it is recommended to treat them as late-winter growers. Their soft leaves make them susceptible to damage, and they may also be prone to mealybugs.

With regards to propagation, this plant can be propagated from both seeds and cuttings.

Seed propagation of Glottiphyllum plants is a relatively straightforward process. The seeds of Glottiphyllum are known to germinate easily and can be sown in autumn in its winter-rainfall habitat, or in early to mid-spring.It is possible to sow them at other times of the year as well, except in very hot weather in the summer. For successful germination, it is important to use well-drained soil and create a warm and humid environment. Do not sow the seeds too deep, as this may prevent them from germinating. Under optimal conditions, the seedlings will grow quickly. Once the seedlings reach a height of 3-5 cm, they can be transplanted into their permanent location.

Glottiphyllum plants can also be propagated through stem cuttings. This method is typically performed towards the end of summer. The cuttings should be taken from the stems and placed in a propagating case with a temperature ranging from 15-21°C. Stem cuttings have the advantage of remaining true to the species, whereas seeds from open pollinated plants in cultivation can be hybrids.