Astroloba rubriflora (L.Bolus) Gideon F.Sm. & J.C.Manning can be distinguished by its matt blue-green leaves; by its relatively small divergence angle that causes the five leaf ranks to arrange either vertically or to spiral in the direction of the generative spiral; the long red secund flowers with red to orange lobes, and perianth segments that are not inflated next to midrib and smooth to the touch. It occurs in nutrient-poor soils on rocky slopes or flats and karroid hills in the Robertson Karoo region of the Western Cape. This species flowers in summer, from December to April.

Astroloba congesta (Salm-Dyck) Uitewaal can be distinguished by its smooth, glossy, unadorned yellow-green leaves; the flowers that are born on very short pedicels, with white or cream lobes, and perianth segments that are not inflated next to the midrib and smooth to the touch. Inflorescences are often branched. It occurs in karroid and valley bushveld vegetation in the Eastern Cape, and has the eastern-most distribution range in genus. This species flowers in late spring, from October to November.

Astroloba robusta P.Reineke ex Molteno, Van Jaarsv. & Gideon F.Sm. can be distinguished by its glossy, grey-green leaves, with whitish margins and keel, the leaf surfaces that occasionally have white spots or elongated tubercules and occasionally dark lines; the flowers that are born sessile or almost sessile on extremely short pedicels, with recurved white lobes, and perianth segments that are not inflated next to midrib and smooth to the touch. The stalk (peduncle) of the unbranched inflorescence is very thick and robust, usually with two large striated bracts. This is the most widespread species in the genus, and occurs on shale flats and rocky undulating slopes across the southern Great Karoo in the Western and Eastern Cape. This species flowers in autumn, winter and spring, from May to October.

Astroloba herrei Uitewaal can be distinguished by its leaves that sometimes have faint longitudinal lines; by its relatively small divergence angle that causes the five ranks of leaves to spiral counter to the direction of the generative spiral; the inflorescence that is unbranched, and the flowers that are born on very long pedicels, with yellow lobes, and perianth segments that are inflated next to the midrib and fairly rough to the touch. It occurs in karroid vegetation both north and south east of the Groot Swartberg Mountains in the Western Cape. This species flowers in late spring, from October to November.