Ledebouria mokobulanensis is easy to maintain in cultivation, although there is no record of it being grown out of its natural habitat, except at the Walter Sisulu National Botanical Garden, where it was grown for purposes of research into the species. Because of its small size, it is most suited to being grown in containers and prefers a well-drained, peaty, loam soil. An ideal potting mixture would include 1 part bark-based potting soil, 1 part peat (or peat alternative such as palm coir, bagasse or fern fibre), 1 part old, well-rotted compost or sifted cattle manure and 2 parts clay loam. The potting medium should provide a slightly acidic soil environment. Watering should be frequent during the summer months (preferably using rainwater if available). The plants prefer a cool, summer climate with good air circulation and the soil should be allowed to remain continuously damp in the growing season. Growing the plants in full sun will ensure an attractive and compact growth habit, as well as optimum leaf colouration. During the dry winter dormancy period, the pots should be watered only very lightly every 2 to 3 weeks to maintain some moisture content in the growth media. Watering can commence towards the end of winter at the onset of warmer temperatures (August–September in South Africa).

Propagation from seed is the best method of producing more plants; the seed must be sown fresh. Sow seeds immediately (do not store them) in spring or summer, in shallow seed trays filled with the sifted potting mixture mentioned above. Spread seed on a firmly tamped surface and lightly cover with the same mixture, approximately 2 mm deep. The trays should be kept in a bright, warm position out of direct sunlight and kept damp until germination has commenced. Seedlings can remain in the seedling tray or planted out as soon as they are large enough to handle. Flowering size bulbs can be attained after 2 to 3 years from germination.