Disa graminifolia is best grown in terracotta pots, which provide optimal drainage and aeration for its root system. Despite its stunning appearance, maintaining this orchid over an extended period can prove to be a challenging endeavour. Best results are obtained when cultivators recreate the conditions in its natural habitat, and incorporate some soil and organic matter sourced from its native environment into the growing medium. This meticulous attention to detail is crucial for ensuring the health and vigour of the plant in cultivation. Incorporating soil from its natural habitat into the growing medium not only provides essential nutrients but also helps to mimic the unique microorganisms and symbiotic relationships present in its native ecosystem. This approach aims to create a harmonious environment that closely resembles the conditions in which Disa graminifolia thrives in the wild.

Disa graminifolia is difficult to propagate, and difficult to keep alive in containers.

Plants can be propagated from seed, although it is very difficult for the home gardener to successfully do so. In habitat, the tiny seeds need to land on suitable soil to be able to form associations mycorrhizae in the soil to obtain nutrients in order to survive. In the nursery they need to be sown under sterile, laboratory conditions on specially formulated growth medium that contains the correct mycorrhizal fungus. To obtain seeds in a garden or laboratory situation, the flowers must be hand-pollinated, by taking pollen from one flower and placing it on the stigma of a different flower. Harvest the capsule as it begins to split and allow it to dry and release the seeds.

Clump-forming disas can be propagated by division. When the daughter plants are fully established, lift the plant just before the growing season begins, in late summer (March). Handle with care, use clean, sterilized tools, gently separate the offsets, and replant immediately in soil that contains the correct mycorrhizal fungus.

Disa graminifolia requires a sandy, acidic growth medium, preferably with some soil from its habitat included. The recommended procedure is to place a 2–3 cm layer of acidic well-rotted compost (such as pine needle compost) at the bottom of the container, and fill up the container with a soil mix of 3 parts coarse, washed river sand mixed with 1 part soil from the habitat (or river sand mixed with habitat soil). Place the container in a position that gets morning sun and afternoon shade or bright light for most of the day. Place it in a protected position that is sheltered from heavy rain and strong wind, and that gets good ventilation, such as a house with open sides and an opaque roof. They should not be allowed to overheat.

Leave the plants in the soil during the dormant period, allow the soil to dry out and store the container in a cool, dry place. Do not lift the tubers, they desiccate very easily and will perish.

Begin watering when the first leaf shoots appear towards the end of autumn. Give them a heavy drench, every 7–10 days and allow the soil to almost dry out completely before the next watering. Use slightly acidic water or rain water, or water that is free of dissolved salts. Tap water should be allowed to stand for 3 hours for the chlorine to evaporate. As soon as the leaves begin to wither, water less frequently and when the plant is dormant let the soil dry out and keep it dry during summer and place the containers in a cool, dry spot.

Feed with hydroponic or liquid organic fertiliser at half the recommended rate, once a month during the active growth period.